Process for preparing biological mammalian implant

专利名称：Process for preparing biological mammalian

implants

发明人：Petr Klement,Gregory J. Wilson,Herman

Yeger

申请号：US07/077753申请日：19870727公开号：US04776853A公开日：19881011

摘要：A process for preparing biological material for implant in mammal'scardiovascular system, respiratory system or soft tissue is disclosed. The processcomprises:

(1) isolating from a suitable donor a desired tissue sample of the biological material;

< P>(2) extracting the tissue sample with an hypotonic buffer solution at a mildalkaline pH, the buffer solution including active amounts of proteolytic inhibitors andantibiotics;

(3) extracting the tissue sample with a buffered solution having a high concentrationof salt, the solution being at a mild alkaline pH and including a non-ionic detergent withprotease inhibitors and antibiotics;

(4) subjecting tissue sample to enzymatic digestion in a buffered saline solution, theenzymes consisting of purified protease- free dioxyribonuclease and ribonuclease;

(5) extracting the tissue sample with an anionic detergent at a mild alkaline pH; and

(6) storing the tissue sample in physiologic buffered solutions.

申请人：HSC RESEARCH DEVELOPMENT CORPORATION

代理机构：Banner, Birch, McKie and Beckett

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